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KD-Validated Anti-Annexin A1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1975
Application
WB, FC, ICC
Uniprot Id
P04083
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-annexin A1 antibody (Cat#ABG1975). Total cell lysates (10 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin A1 antibody (Cat#ABG1975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-annexin A1 antibody (Cat#ABG1975). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-annexin A1 antibody (Cat#ABG1975, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Annexin A1 expression in HepG2 cells using anti-Annexin A1 antibody (Cat#ABG1975, 1:2,000). Green, isotype control; red, Annexin A1.
  • Immunocytochemical staining of HepG2 cells with anti-Annexin A1 antibody (Cat#ABG1975, 1:1,000). Nuclei were stained blue with DAPI; Annexin A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1975-100 3 days 100 µL $499.00
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ABG1975-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Annexin A1 Rabbit Monoclonal Antibody
Accession (Primary)P04083
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6815
Immunogen
Recombinant protein of human Annexin A1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 39 kDa, observed, 33 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P04083 )

Gene NameANXA1
Other Gene Names
ANXA1; Annexin A1; ANX1; LPC1; Phospholipase A2 Inhibitory Protein; Chromobindin-9; Calpactin II; Calpactin-2; Annexin-1; Epididymis Secretory Sperm Binding Protein; Annexin I (Lipocortin I); Lipocortin I; Annexin I; P35
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