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KD-Validated Anti-CHMP2B Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1146
Application
WB, FC, ICC
Uniprot Id
Q9UQN3
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CHMP2B antibody (Cat#ABG1146). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CHMP2B antibody (Cat#ABG1146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CHMP2B antibody (Cat#ABG1146). CHMP2B expression in wild type (WT) and CHMP2B shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CHMP2B antibody (Cat#ABG1146, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody (Cat#ABG1146, 1:2,000). Green, isotype control; red, CHMP2B.
  • Immunocytochemical staining of HepG2 cells with CHMP2B antibody (Cat#ABG1146, 1:1,000). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1146-100 3 days 100 µL $499.00
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ABG1146-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-CHMP2B Rabbit Monoclonal Antibody
Accession (Primary)Q9UQN3
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3070
Immunogen
A synthesized peptide derived from CHMP2B
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 24 kDa , observed, 28 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9UQN3 )

Gene NameCHMP2B
Other Gene Names
CHMP2B; Charged Multivesicular Body Protein 2B; CHMP2.5; VPS2B; Charged Multivesicular Body Protein 2b; Chromatin Modifying Protein 2B; DKFZP564O123; Vacuolar Protein-Sorting-Associated Protein 2-2; Vacuolar Protein Sorting-Associated Protein 2-2; VPS2 Homolog B (S. Cerevisiae); Chromatin-Modifying Protein 2b; VPS2 Homolog B; FTDALS7; HVps2-2; VPS2-2; CHMP2b; Vps2-2; ALS17; DMT1
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