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KD-Validated Anti-IFIH1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1058
Application
WB, ICC
Uniprot Id
Q9BYX4
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-IFIH1 antibody (Cat#ABG1058). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IFIH1 antibody (Cat#ABG1058, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-IFIH1 antibody (Cat#ABG1058). IFIH1 expression in wild type (WT) and IFIH1 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-IFIH1 antibody (Cat#ABG1058, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-IFIH1 antibody (Cat#ABG1058, 1:1,000). Nuclei were stained blue with DAPI; IFIH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1058-100 3 days 100 µL $499.00
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ABG1058-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-IFIH1 Rabbit Monoclonal Antibody
Accession (Primary)Q9BYX4
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB6085
Immunogen
A synthesized peptide derived from human MDA5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 117 kDa, observed, 140 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,00

Target Information: ( Q9BYX4 )

Gene NameIFIH1
Other Gene Names
IFIH1; Interferon Induced With Helicase C Domain 1; MDA-5; MDA5; Helicard; IDDM19; Interferon-Induced Helicase C Domain-Containing Protein 1; Clinically Amyopathic Dermatomyositis Autoantigen 140 KDa; Melanoma Differentiation-Associated Protein 5; Melanoma Differentiation-Associated Gene 5; RNA Helicase-DEAD Box Protein 116; Murabutide Down-Regulated Protein; Helicase With 2 CARD Domains; RIG-I-Like Receptor; CADM-140 Autoantigen; RLR-2; Hlcd; Interferon-Induced With Helicase C Domain Protein 1; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide; EC 3.6.4.13; SGMRT1; IMD95; RH116; AGS7; HLCD
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