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KD-Validated Anti-ISG15 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1567
Application
WB, ICC
Uniprot Id
P05161
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ISG15 antibody (Cat#ABG1567). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ISG15 antibody (Cat#ABG1567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ISG15 antibody (Cat#ABG1567). ISG15 expression in wild-type (WT) and ISG15 shRNA knockdown (KD) HepG2 cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ISG15 antibody (Cat#ABG1567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-ISG15 antibody (Cat#ABG1567, 1:1,000). Nuclei were stained blue with DAPI; ISG15 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1567-100 3 days 100 µL $499.00
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ABG1567-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ISG15 Rabbit Monoclonal Antibody
Accession (Primary)P05161
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB6460
Immunogen
A synthesized peptide derived from human ISG15
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 18 kDa , observed , 15 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P05161 )

Gene NameISG15
Other Gene Names
ISG15; ISG15 Ubiquitin Like Modifier; UCRP; IFI15; G1P2; Interferon, Alpha-Inducible Protein (Clone IFI-15K); Ubiquitin Cross-Reactive Protein; Ubiquitin-Like ProteinISG15; HUCRP; IP17; Interferon-Induced 17-KDa/15-KDa Protein; Interferon-Stimulated Protein, 15 KDa; Interferon-Induced 15 KDa Protein; Interferon-Induced 17 KDa Protein; IMD38
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