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Simian Testo(Testosterone) ELISA Kit

Simian Testo(Testosterone) ELISA Kit

 
Catalog #
EKEL12780MK
Species
Simian
Sensitivity
0.11 ng/mL
Assay Range
0.32-20 ng/mL
Sample type(s)
Serum, plasma, other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL12780MK-96 2 weeks 96T $470.00
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EKEL12780MK-48 2 weeks 48T $335.00
EKEL12780MK-96x5 2 weeks 96T*5 $2,000.00
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Product Overview

NameSimian Testo(Testosterone) ELISA Kit
Reaction Duration2.5h
Target SpeciesSimian
ReactivitySimian
Sensitivity0.11 ng/mL
Assay Range0.32-20 ng/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma, other biological fluids
Other Names
T; Testesteron; Testeronun
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian Testo. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian Testo. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian Testo in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (ng/mL)ODCorrected OD
20.000.199
10.000.397
5.000.586
2.500.895
1.251.225
0.631.592
0.321.863
0.002.115
Standard curve: The concentration of Testo (ng/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant Testo and the recovery rates were calculated by comparing the measured value to the expected amount of Testo in samples.

MatrixRecovery rangeAverage
Serum (n=5)90-105%98%
EDTA plasma (n=5)81-97%89%
Heparin plasma (n=5)95-107%101%

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Research Use Only
For research use only. Not intended for diagnostic or therapeutic procedures.