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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL15149MK-96 | 2 weeks | 96T | $735.00 |
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| EKEL15149MK-48 | 2 weeks | 48T | $515.00 | |||
| EKEL15149MK-96x5 | 2 weeks | 96T*5 | $3,125.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Simian HMGB-1(High Mobility Group Protein B1) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Reaction Duration | 3.5h | |||||||||||||||||||||||||||
| Target Species | Simian | |||||||||||||||||||||||||||
| Reactivity | Simian | |||||||||||||||||||||||||||
| Sensitivity | 0.064 ng/mL | |||||||||||||||||||||||||||
| Assay Range | 0.16-10 ng/mL | |||||||||||||||||||||||||||
| Assay type | Sandwich | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein; HMG1; High Mobility Group Protein 1 | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Simian HMGB-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian HMGB-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Simian HMGB-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian HMGB-1 in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant HMGB-1 and the recovery rates were calculated by comparing the measured value to the expected amount of HMGB-1 in samples.
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