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Simian MTL(Motilin) ELISA Kit

Simian MTL(Motilin) ELISA Kit

 
Catalog #
EKEL15211MK
Species
Simian
Sensitivity
27.62 pg/mL
Assay Range
93.75-6000 pg/mL
Sample type(s)
Serum, plasma and other biological fluids
 
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 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
EKEL15211MK-96 2 weeks 96T $575.00
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EKEL15211MK-48 2 weeks 48T $400.00
EKEL15211MK-96x5 2 weeks 96T*5 $2,435.00
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Product Overview

NameSimian MTL(Motilin) ELISA Kit
Reaction Duration2h
Target SpeciesSimian
ReactivitySimian
Sensitivity27.62 pg/mL
Assay Range93.75-6000 pg/mL
Assay typeCompetitive Inhibition
Sample type(s)Serum, plasma and other biological fluids
Other Names
MLN; MAP; Promotilin; Motilin-associated peptide
Principle of the Assay
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian MTL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian MTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian MTL in the samples is then determined by comparing the OD of the samples to the standard curve.
Standard Curve
Concentration (pg/mL)ODCorrected OD
6000.000.189
3000.000.386
1500.000.611
750.000.856
375.001.197
187.501.592
93.752.197
0.002.912
Standard curve: The concentration of MTL (pg/mL) plotted against Corrected OD.
Precision
AssayCVMethod
Intra-assay (precision within an assay)< 8%Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay (precision between assays)< 10%Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
Recovery

Matrices listed below were spiked with certain level of recombinant MTL and the recovery rates were calculated by comparing the measured value to the expected amount of MTL in samples.

MatrixRecovery rangeAverage
Serum (n=5)87-99%93%
EDTA plasma (n=5)79-97%88%
Heparin plasma (n=5)89-103%96%

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Research Use Only
For research use only. Not intended for diagnostic or therapeutic procedures.