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| Catalog # | Availability | Size | Quantity | Unit Price | Save For Later Wish List | |
|---|---|---|---|---|---|---|
| EKEL15213MK-96 | 2 weeks | 96T | $575.00 |
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| EKEL15213MK-48 | 2 weeks | 48T | $400.00 | |||
| EKEL15213MK-96x5 | 2 weeks | 96T*5 | $2,435.00 |
Product Overview | ||||||||||||||||||||||||||||
| Name | Simian TRH(Thyrotropin Releasing Hormone) ELISA Kit | |||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Reaction Duration | 2h | |||||||||||||||||||||||||||
| Target Species | Simian | |||||||||||||||||||||||||||
| Reactivity | Simian | |||||||||||||||||||||||||||
| Sensitivity | 2.7 pg/mL | |||||||||||||||||||||||||||
| Assay Range | 7.82-500 pg/mL | |||||||||||||||||||||||||||
| Assay type | Competitive Inhibition | |||||||||||||||||||||||||||
| Sample type(s) | Serum, plasma and other biological fluids | |||||||||||||||||||||||||||
| Other Names | ||||||||||||||||||||||||||||
| TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor | ||||||||||||||||||||||||||||
| Principle of the Assay | ||||||||||||||||||||||||||||
| This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Simian TRH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Simian TRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Simian TRH in the samples is then determined by comparing the OD of the samples to the standard curve. | ||||||||||||||||||||||||||||
| Standard Curve | ||||||||||||||||||||||||||||
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| Precision | ||||||||||||||||||||||||||||
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| Recovery | ||||||||||||||||||||||||||||
Matrices listed below were spiked with certain level of recombinant TRH and the recovery rates were calculated by comparing the measured value to the expected amount of TRH in samples.
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